Document Type : Original Article


1 Department of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants P.O. CIMAP, Lucknow UP-226 015, India

2 Department of Biotechnology, CSIR-Central Institute of Medicinal and Aromatic Plants P.O. CIMAP, Lucknow UP-226 015, India

3 Department of Analytical Chemistry, CSIR-Central Institute of Medicinal and Aromatic Plants P.O. CIMAP, Lucknow UP-226 015, India


Sixteen accessions belonging to six species of Ocimum from Uttar Pradesh (12), Andhra Predesh (3) and Marashtra (1) states of India were screened for ursolic acid and higher oil yields. A considerable amount of genetic variability in morphometric traits was recorded in all accessions. It was realized that both the estimate of heritability broad sense in percent (^h² %) and the corresponding genetic advance (GA) were high for oil yield (98.80 and 120.35) and herb yield/plant (93.52 and 89.27). However, high genetic heritability and moderate genetic advance expressed by days to flower 50% (95.13 and 49.07) followed by plant height (93.54 and 52.17) and ursolic acid yield (99.99 and 78.14), respectively. The ursolic acid expressed ^h² % and low GA (99.99 and 0.362) followed by oil content, leaf/stem ratio and ursolic acid content (99.99, 0.362). Correlation coefficients among the ten traits indicated that leaf/stem ratio was positively highly and significantly correlated with oil content (0.743**, 0.710**) and oil yield (0.700**, 0.676**). Leaf/stem ratio was also positively and significantly correlated with ursolic acid yield (0.551*, 0.536*). The herb yield was highly positive and significantly correlated with oil yield (0.790**, 0.772**). The oil content also had a high and significant correlation with oil yield (0.877**, 0.867**). In addition, similar to the aforementioned correlations, the ursolic acid was significantly and positively correlated with ursolic acid yield (0.966**, 0.965**) at both genotypic and phenotypic level. The path coefficient revealed that the highest direct contribution to ursolic acid was made by ursolic acid content (0.904) and herb yield (0.264). The oil content had the maximum positive indirect effect (0.471) to the ursolic acid content. The residual effect value was found to be 0.0248. Ocimum accessions CIM Ayu followed by Sel-1, CIM Jyoti, CIM Snigdha and CIM Shurabhi may be exploited for commercial cultivation.

Graphical Abstract

Quantification of ursolic acid, correlations and contribution by other traits towards accumulation of ursolic acid in six Ocimum species


Main Subjects

Anandjiwala, S., Kolola, J., Rajani, M., 2006. Quantification of eugenol, luteolin, ursolic acid, and oleanolic acid in black (Krishna tulsi) and green (Sri tulsi) of Ocimum sanctum Linn. using high-performance thin-layer chromatography. J. AOAC Int. 89, 1467-1474.
Clevenger, J F., 1928 Apparatus for determination of volatile oils. J. Am. Pharm. Assoc. 17, 345.
Dewey, D.R. Lu, K.H., 1959. A correlation and path coefficient analysis components of crested wheat grass seed production. Agron. J. 51, 515-518.
Gnoatto, S.C.B., Schenkel, E.P., Bassani, V.L., 2005. HPLC method to assay total saponins in Ilex paraguariensis aqueous extract. J. Braz. Chem. Soc. 16(4), 723-725.
Guenther, E. 1949. The essential oils VIII Roberts E. Krieger Publ. Co. Malabar, Florida, 399-433.
Silva, M.G.V., Vieira, L.G.P., Mendes, F.N.P., Albuquerque, L.L., Santos, Rogério N.D., Silva, F.O., Morais S.M. 2008. Variation of ursolic acid content in eight Ocimum species from northeastern Brazil. Molecules 13(10), 2482-2487.
Heath, H.B. 1981. Source Book of Flavours. AVI. West Port, CT, 222-223.
Huang, M.T., Ho, G.T., Wang, Z.Y., Ferraro, T., Lou, Y.R., Stauber, K., Ma, W., Georgiadis, C., Laskin, J.D., Conney, A.H., 1994. Inhibition of skin tumorigenesis by rosemary and its constituents carosol and ursolic acid. Cancers Res. 54, 701-708.
Lal, R.K., 2014. Breeding for new chemotypes with stable high essential oil yield in Ocimum. Ind. Crop Prod. 59, 41-49.
Lal, R. K., Sharma, J. R., Misra, H. O., Sharma, S., Naqvi, A.A., 2001. Genetic variability and relationship in quantitative and qualitative traits of Java Citronella (Cymbopogon winterianus, Jowitt). J. Essent. Oil Res. 13,158-162.
Liu, J., 2005. Oleanolic acid and ursolic acid: Research perspectives. J. Ethnopharmacol. 100, 92-94.
Panse, V.G., Sukhatme, P.V., 1967. Statistical methods for agricultural workers. 2nd Ed. Indian Council of Agricultural Research, New Delhi.
RazborŠek, M.I., Vončina, D.B., Deleček, V., Vončina, E., 2008. Determination of oleanolic, betulinic, andursolic acid in Lamiaceae and mass spectral fragmentation of their trimethylsilylated derivatives. Chromatographia. 67, 433-440.
Reuveni, R., Fleisher, A. Putieusky, E., 1984. Fungistatic activity of essential oils from Ocimum basilicum chemotypes. Phytopath Z. 110, 20-22.
Silva, M.G.V., Matos, F.J.A., Machado, M.L.L., Craveiro, A.A., 2013. Essential oils of Ocimum basilicum L., O. basilicum var minimum and Ocimum basilicum var purpurascens grown in Northeastern Brazil. Flav. Frag. J. 18(1), 13-14.
Singh, R.K., Chaudhary, B.D., 1979. Variance and covariance analysis. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publisher, New Delhi India pp, 57.
Simon, J., Peifer, M., Bender, W., O'Connor, M., 1990. Regulatory elements of the bithorax complex that control expression along the anterior-posterior axis. EMBO J. 9(12), 3945--3956.
Sobti, S.N., P. Pushpangadan, Atal., C.K., 1982. Clocimum: A New Hybrid Strain of Ocimum guatissimum As a Potential Source of Clove Type Oil, Rich in Eugene In: Atal, C.K. and B.M. Kapur. Cultivation and Utilization of Aromatic Plants. Reg. Res. Lab., Jammu Tawi, India, 473-480.
Švccovȧ, E., Ncugcbaucrovȧ, J., 2010. A study of 34 cultivars of basil (Ocimum L.) and their morphological, economic and biochemical characteristics, using standardized descriptors. Acta Univ. Sapientiae. Alimentaria. 3, 118-135.
Zupancic, A., 2001. Tropical anti-inflammatory activity of Salvia officinalis L. leaves: the relevance of ursolic acid. J. Ethnopharmacol. 75, 125-132.