Leucas aspera (willd.) L.: Antibacterial, antifungal and mosquitocidal activities

Document Type: Original Article


1 Post Graduate & Research Department of Zoology, Vivekananda College, Affiliated to Madurai Kamaraj University (MKU), Tiruvedakam West, Madurai District – 625 234, Tamil Nadu, India

2 Department of Zoology, Saiva Bhanu Kshathriya College (SBK), Affiliated to Madurai Kamaraj University (MKU), Aruppukkottai – 626 101, Virudhunagar District, Tamil Nadu, India

3 Optimurz Bio and IT Solutions, Shenoy Nagar West, Chennai-600030, Tamil Nadu, India


The various organic extracts from the leaves of Leucas aspera were screened for their antibacterial, antifungal and larvicidal activities against selected bacterial, fungal strains and mosquito larvae of Culex quinquefasciatus. Antimicrobial activity was carried out using disc-diffusion method and MIC of the extract was tested by the broth micro-dilution method. The results revealed that all the extracts showed antibacterial and antifungal activities against selected microbes at 1, 2.5, 5 and 10 mg/disc concentrations. The maximum zone of inhibitions were recorded in dichloromethane (DCM) leaf extract of L. aspera against S. aureus (23.4 ± 2.90 mm), E. coli (20.3 ± 1.56 mm), B. subtilis (17.1 ± 2.04 mm), P. aeruginosa (16.5 ± 1.05 mm), P. vulgaris (16.1 ± 2.56 mm) and K. pneumonia (15.1 ± 3.66 mm) at 10 mg/disc concentration. The DCM extract of L. aspera exhibited significant growth inhibition against T. viride (29.2 ± 2.00 mm), C. albicans (24.4 ± 0.80 mm), A. flavus (22.8 ± 0.36 mm) and E. floccosum (19.5 ± 2.17 mm). The minimum inhibitory concentration (MIC) ranges between 75.5-425.5 µg/mL and 125-425 µg/mL against bacterial and fungal pathogens, respectively. In addition, DCM extracts of L. aspera showed 100% larvicidal activity against C. quinquefasciatus at 1000 ppm concentration. The biological activities could contribute to the medicinal properties of the plants, and also provide more scientific authentication of traditional medicinal plants to fight against the various infectious diseases

Graphical Abstract

<i>Leucas aspera</i> (willd.) L.: Antibacterial, antifungal and mosquitocidal activities


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