Document Type : Original Article


1 Department of Natural Product Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India

2 Department of Chemistry, Sreekrishna College, Ariyanoor, Guruvayoor, Thrissur, Kerala, India

3 Department of Chemistry, Devanga Arts College, Arupukottai, Tamil Nadu, India


Alpinia galanga and Alpinia calcarata are two important aromatic medicinal plants belong to the family Zingiberaceae. In the present study, dried rhizomes of A. galanga and A. calcarata were extracted with water and then fractionated with different solvents. Among the various solvent fractions, ethyl acetate (EA) fraction of both the plants showed high polyphenolic content with better antioxidant activity. This polyphenol rich EA fraction is also screened for its antidiabetic and anti-inflammatory potential using in vitro assays. Results showed that EA fractions of these two plants had significant antihyperglycemic activity by inhibiting carbohydrate digesting enzymes (α-amylase and α-glucosidase) and by inhibiting glycation reaction. Inhibitory potential of EA fractions of both the plant against protein denaturation and xanthine oxidase validates its anti-inflammatory potential. Moreover, EA fractions also exhibited significant LDL oxidation inhibition .The observed antidiabetic and anti-inflammatory properties of A. galanga and A. calcarata could be due to the synergic effect of polyphenols such as gallic acid and ellagic acid as well as bioactive alkaloid, berberine.

Graphical Abstract

Evaluation of antidiabetic, anti-inflammatory and LDL oxidation inhibitory potential of Alpinia galanga and Alpinia calcarata -An in vitro study


Main Subjects

Ali, H., Houghton, P.J., Soumyanath, A., 2006. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol.107, 449-455.
Apostolidis, E., Kwon, Y.I., Shetty, K., 2007. Inhibitory potential of herb, fruit, and fungal- enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Inn. Food Sci. Emer. Technol. 8, 46-54.
Arom, J., 2005. In vitro antiglycation activity of arbutin. Naresuan Univ. J. 3(2), 35-41.
Asgarpanah, J., Amin, G., Parviz, M., 2011. In vitro antiglycation activity of Eremurus persicus (Jaub. Et Sp.) Boiss. Afr. J. Biotechnol. 10(54), 11287-11289.
Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.-M., Linder, T., Wawrosch, C., Uhrin, P., Temml, V., Wang, L., Schwaiger, S., Heiss, E.H., 2015. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol. Adv. 33, 1582-1614.
Berry, C.E., Hare, J.M., 2004. Xanthine oxidoreductase and cardiovascular disease: the molecular mechanisms and pathophysiological implications. J. Physiol. 555(3), 589-606.
Burke, A., Smyth, E., Fitzgerald, G.A., 2006. Analgesic-antipyretic agents: Pharmacotherapy of gout. In: Brunton, L. L., Lazo, J. S., Parker, K. L. (Ed.). The pharmacological basis of therapeutics, 11th Ed. McGraw Hill Medical Publishing Division, New York, p. 706-710.
Chandra, S., Chatterjee, P., Dey, P., Bhattacharya, S., 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific J. Trop. Biomed. 2(1), S178-S180.
Das, S., Datta, R., Nandy, S., 2012. Phytochemical screening and evaluation of anti-inflammatory activity of methanolic extract of Abroma augusta Linn. Asian Pacific J. Trop. Disease. S114-S117.
Duan, X.J., Zhang, W.W., Li, X.M., Wang, B.G., 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga. Polysiphonia urceolata. Food Chem. 95, 37-43.
 Gothai, S., Ganesan, P., Park, S. Y., Fakurazi, S., Choi, D.G., Arulselvan, P., 2016. Natural phyto-bioactive compounds for the treatment of type 2 diabetes: Inflammation as a target. Nutrients 8, 1-28.
Halliwell, B., 2001. Role of free radicals in the neurodegenerative diseases: therapeutic implications for antioxidant treatment.Drugs Aging 18, 685-716.
Ivanovska, N., Philipov, S., 1996. Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int. J. Immuno. Pharmacol.18, 553-561.
Jia, Z., Mengcheng, T., Wu, J., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555-559.
Kim, Y.M., Jeong, Y.K., Wang, M.H., Lee, W.Y., Rhee, H.I., 2005. Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition 21(6), 756-761.
Kinsella, J.E., Frankel, E., German, B., Kanner, J., 1993. Possible mechanism for the protective role of antioxidants in wine and plant foods. Food Technol. 47, 85-89.
Kong, W., Wei, J., Abidi, P., Lin, M., Inaba, S., Li, C., Wang, Y., Wang, Z., Si, S., Pan, H., Wang, S., Wu, J., Wang, Y., Li, Z., Liu, J., Jiang, J.D., 2004. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat. Med. 10, 1344-1351.
Kotamballi, N., Chidambara, M., Ravendra, P.S, Jayaprakasha, K.G., 2002. Antioxidant activities of grape (Vitis vinifera) pomace extracts. J. Agric. Food Chem. 50, 5909-5914.
 Krentz, A.J., Bailey, C.J., 2005. Oral antidiabetic agents. Current role in type 2 diabetes mellitus. Drugs 65, 385-411.
Meek, I.L., Van de Laar MAF, J., Vonkeman, H.E., 2010. Non-steroidal anti-inflammatory drugs: An overview of cardiovascular risks. Pharmaceuticals3, 2146-2162.
Miguel, M.G., 2010. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules 15, 9252-9287.
Nagashekhar, M., Shivaprasad, H.N., 2006. An important medicinal plant: A review Biomedicine 1(1), 63-68.
Nakagawa, T., Yokozawa, T., Terasawa, K., Shu, S., Juneja, L.R., 2002. Protective activity of green tea against free radical and glucose mediated protein damage. J. Agric. Food Chem. 50, 2418-2422.
Nampoothiri, S.V, Esakkidurai, T., Pitchumani, K., 2015. Identification and quantification of phenolic compounds in Alpinia galanga and Alpinia calcarata and its relation to free radical quenching properties-a comparative study.J. Herbs Spices Med. Plants. 21, 140-147.
Nampoothiri, S.V., Esakkidurai, T., Pitchumani, K., 2017. Isolation and quantification of berberine alkaloid from Alpinia galanga and Alpinia calcarata.Int. J. Pharma Sci. Res. 8(6), 97-104.
Nickavar, B., Yousefian,N., 2009. Inhibitory effects of six Allium species on α-amylase enzyme activity. Iran. J. Pharm. Res. 8(1), 53-57.
Pathak, K., Das, R.J., 2013. Herbal medicine- A rational approach in health care system. Int. J. Herb. Med. 1(3), 86-89.
Prathapan, A., Singh, M.K., Anusree, S.S., Sobankumar, D.R., Sundaresan, A., Raghu, K.G., 2011. Antiperoxidative, free radical scavenging and metal chelating and metal chelating activities of Boerhaavia diffusa L. J. Food Biochem. 35, 1548-1554.
Prathapan, A., Cherian, O.L, Nampoothiri, S.V, Mini, S, Raghu, K.G., 2011. In vitro antiperoxidative, free radical scavenging and xantine oxides inhibitory potential of ethyl acetate fraction of Saraca asoka flowers. Nat. Prod. Res. 25(3), 298-309.
 Prathapan, A., Nampoothiri, S.V., Mini, S., Raghu, K.G., 2012. Antioxidant, antiglycation and inhibitory potential of Saraca ashoka flowers against the enzymes linked to type 2 diabetes and LDL oxidation. Eur. Rev. Med. Pharmacol. Sci. 16, 57-65.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolouration assay. Free Radic. Biol. Med. 26, 1231-1237.
Schafer, A., Hogger, P., 2007. Oligomericprocyanidins of French maritime pine bark extract (Pycnogenol) effectively inhibit alpha-glucosidase. Diabetes Res. Clin. Pract.77, 41-46.
Shabir, G.A., 2003. Validation of high performance liquid chromatography methods for pharmaceutical analysis understanding differences and similarities between validation requirements of US Food and Drug Administration, the US Pharmacopeia and International Conference of Harmonization. J. Chromatogr. A 987, 57-66.
Shimada, K.K, Fujikawa, K., Yahara, K., Nakamura, T., 1992. Antioxidative properties of xanthan on the autooxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 40, 945-948.
Shinde, J., Taldone, T., Barletta, M., Kunapararhyju, N., Hu, B., Kumar, S., 2008. α-Glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in in vitro and in Goto-Kakizaki (GK) rats. Carbohydr. Res. 343, 1278-1281.
 Shivkanya, J., Nitin, I., Dinesh, S., Neeraj, F., Mohamad, A., 2009.Isolation of galangogalloside from rhizomes of Alpinia galanga. Int. J. Green Pharm. 3(2), 144-147.
Sostres, C., Gargallo, C.J., Lanas, A., 2013. Nonsteroidal anti-inflammatory drugs and upper and lower gastrointestinal mucosal damage. Arthritis Res. Ther. 15(3), 1-8.
Sun, B., Jorge, M., Silva, R.D, Spraner, S., 1998. Critical factors of vanillin assay for catechins and proanthocyanidins. J. Agric. Food Chem. 46, 4267-4274.
Wiztum, J.L., Steinberg, D., 1991. Role of oxidized low density lipoprotein in atherogenesis. J. Clin. Invest. 88, 1785-1792.
Yan-xia, N., An-qiang, L., Yun-feng, G., Wei-hong W., Ya-gui, S., 1995. Therapeutic effect of berberine on 60 patients with non-insulin dependent diabetes mellitus and experimental research. Chin. J. Int. Trad. Med. 1, 91-95.