Intra-specific differentiation, genetic variability and their prospect for exploitation in medicinally important plant Black henbane (Hyoscyamus niger L.)

Document Type: Original Article


Department of Genetics and Plant Breeding, CSIR - Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, Uttar Pradesh (India) 226015


Black henbane (Hyoscyamus niger L.) is frequently cultivated as a rich source of tropane alkaloids particularly hyoscyamine and hyoscine (Scopolamine) which are extracted from its whole aerial herb including dried leaves and flowering tops. These valuable natural compounds are widely used in modern medicinal preparations as well as pharmaceutical industries in India and around the world. In the present study, genetic divergence among twenty-nine accessions of Hyoscyamus niger L. was evaluated for the nature along with amount of genetic diversity for eleven economic traits. Accordingly, all the twenty-nine accessions were grouped into eight diverse clusters (cluster I-VIII). The enormous diversity was also indicated by the wide range of D²-values (4.032 to 544.535). A number of unique accessions HN-30, HN-31, HN-15, HN-22-Y, and HN-29-Y were identified that may be interesting genotypes for the genetic improvement of the different morphometric and tropane alkaloid traits. Therefore, these accessions can be further exploited in future hybridization programs for the development of a new variety/cultivar for commercial productions.

Graphical Abstract

Intra-specific differentiation, genetic variability and their prospect for exploitation in medicinally important plant Black henbane (Hyoscyamus niger L.)


Anonymous, 1959. The Wealth of India: Raw materials. CSIR, New Delhi, India. Hyoscyamus niger.5, 279-292.

Bahmanzadegan, A., Sefidkon, F., Sonboli, A., 2010. Determination of hyoscyamine and scopolamine in four Hyoscyamus species from Iran. Iranian J. Pharm. Res. 8(1), 65-70.

Banani, N., Mehrafarin, A., HosseiniMazinani, S.M., 2017. Changes of tropane alkaloids in Black Henbane (Hyoscyamus niger L.) in response to different types of nitrogenous fertilizers. J. Med. Plants 16, 56-67.

Begum, S.A., Sahai, M., Ray, A.B., 2010. Non-conventional Lignans: Coumarinolignans, Flavonolignans, and Stilbenolignans, in: Fortschritte Der ChemieOrganischerNaturstoffe / Progress in the Chemistry of Organic Natural Products, Fortschritte Der Chemie Organischer Naturstoffe/Progress in the Chemistry of Organic Natural Products. Springer, Vienna, 93, pp. 1-70.

Camilo, C.J., Alves Nonato, C.d.F., Galvão-Rodrigues, F.F., Costa, W.D., Clemente, G.G., Sobreira Macedo, M.A.C., Galvão Rodrigues, F.F., da Costa, J.G.M., 2017. Acaricidal activity of essential oils: a review. Trends Phytochem. Res. 1(4), 183-198.

Chaudhary, B.D., Singh, R.K., 1985. Biometrical methods in quantitative genetic analysis. Kalyani, New Delhi, Revised Edition, Classificatory Anal. 12, 224-252.

Cromwell, B.T., 1955. The Alkaloids, in: Modern Methods of Plant Analysis / ModerneMethoden Der Pflanzenanalyse, Modern Methods of Plant Analysis/ModerneMethoden Der Pflanzenanalyse. Springer, Berlin, Heidelberg, pp. 367-516.

Ganesan, K., Xu, B., 2017. Ethnobotanical studies on folkloric medicinal plants in Nainamalai, Namakkal District, Tamil Nadu, India. Trends Phytochem. Res. 1(3), 153-168.

Gupta, A.P., Gupta, M.M., Kumar, S., 1999. Estimation of tropane alkaloids using high performance thin layer chromatography. J. Indian Chem. Soc. 76, 277-278.

Kamada, H., Okamura, N., Satake, M., Harada, H., Shimomura, K., 1986. Alkaloid production by hairy root cultures in Atropabelladonna. Plant Cell Rep. 5, 239-242.

Kumar, Y., Prakash, O., Tripathi, H., Tandon, S., Gupta, M.M., Rahman, L.U., Lal, R.K., Semwal, M., Darokar, M.P., Khan, F., 2018. AromaDb: A database of medicinal and aromatic plant’s aroma molecules with phytochemistry and therapeutic potentials. Front. Plant Sci. 9, 1-11.

Lal, R.K., Gupta, P., Sarkar, S., 2018a. Phylogenetic relationships, path and principal component analysis for genetic variability and high oil yielding clone selection in Vetiver (Vetiveria zizanioides L.) Nash. J. Plant Genet. Breed. 2(1), 105-113.

Lal, R.K., Chanotiya, C. S., Dhawan, S.S., Gupta, P., Sarkar, S., 2018b. Genotypic and morphological appearance of the traits in relation to genetic diversity of essential oil yield in Vetiver Grass (Chrysopogon zizanioides Roberty). ASAG 2(8), 62-72.

Lal, R.K., Khanuja, S.P.S., Mishra, H.O., 2005. Induced heterotic genetic diversity in black henbane (Hyoscyamus niger L.). Indian J. Genet. Plant Breed. 65, 63-64.

Lal, R.K., Sharma, J.R., Sharma, S., 2000. Genetic diversity in germplasm of isabgol (Plantago ovate Forsk.). J. Herbs Spices Med. Plants 6, 73-80.

Mahalanobis, P.C., 1936. On the Generalized Distance in Statistics. Gen. Distance Stat. 49-55.

Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.  
Mohammadhosseini, M., 2017. The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Ind. Crops Prod. 105, 164-192.

Moharrami, F., Hosseini, B., Sharafi, A., Farjaminezhad, M., 2017. Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture of Hyoscyamus reticulatus L. elicited by iron oxide nanoparticles. In Vitro Cell. Dev. Biol. 53, 104-111.

Panda, H., 2002. Medicinal Plants Cultivation and Their Uses. National Institute Of Ind. Re.

Nunes, H.S., Miguel, M.G., 2017. Rosa damascena essential oils: a brief review about chemical composition and biological properties. Trends Phytochem. Res. 1(3), 111-128.

Panse, V.G., Sukhatme, PandurangVasudeo, Shaw, 1967. A Handbook of Statistics for Use in Plant Breeding, Frederick John Freshwater, Problems, Agricultural. Statistical Methods for Agricultural Workers, New Delhi: Indian Council of Agricatural Research, p. 2.

Popova, V., Ivanova, T., Stoyanova, A., Georgiev, V., Hristeva, T., Nikolova, V., Docheva, M., Nikolov, N., Damianova, S., 2018. Phytochemicals in leaves and extracts of the variety “Plovdiv 7” of Bulgarian oriental tobacco (Nicotiana tabacum L.). Trends Phytochem. Res. 2(1), 27-36.

Rao, C.R., 1952. Advanced Statistical Methods in Biometric Research. John Wiley and Sons, Inc., New York.

Schläppi, M., 2011. Genetic and physiological analysis of biennialism in Hyoscyamus niger. Plant Biol. 13, 534-540.

Sharma, J.R., Lal, R.K., Gupta, M.M., Mishra, H.O., Sharma, S., 2001. Inheritance of biomass and crude drug content in Black henbane (Hyoscyamus niger L.). J. Herbs. Spices Med. Plants 7, 75-84.

Sharma, J.R., Lal, R.K., Mishra, H.O., Srikant, S., 1992. Induced polygenic changes occurring simultaneously with major genes changes in Hyoscyamus niger L. The App. Genet. 85(4), 445-450.

Sobha, K., Dumpala, P., Dachepalli, H.P., Anantha, R.K., 2018. Understanding the phytochemical constitution, antioxidant potential and spectral characteristics of aqueous extracts of the chosen leafy vegetables from south India. Trends Phytochem. Res. 2(2), 111-118.

Sreeramu, B.S., 2004. Cultivation of Medicinal and Aromatic Crops. Universities Press.

Srivastava, S., Lavania, U.C., 1987. Karyomorphology and DNA amount in relation to chiasma formation in Hyoscyamus niger L. Nucl. India 30, 94-98.

Supria, K.B., Szoke, E., Laszlo, I., Kursinszki, L., (2004). Investigation of tropane alkaloids in genetically transformed Atropa belladonna L. cultures, Chromatographia 60, 555-559.

Thul, S., Lal, R.K., Shasany, A., Darokar, M., Gupta, A., Gupta, M., Verma, R.K., Khanuja, S.P.S., 2009. Estimation of phenotypic divergence in a collection of Capsicum species for yield-related traits. Euphytica 168, 189-196.

Venditti, A., Frezza, C., Serafini, I., Pulone, S., Scardelletti, G., Sciubba, F., Bianco, A., Serafini, M., 2018. Chemical profiling of the fruits of Styrax officinalis L. from Monti Lucretili (Latium region, Central Italy): Chemotaxonomy and nutraceutical potential. Trends Phytochem. Res. 2(1), 1-12.

Whitson, T.D., L.C. Burrill, S.A., Dewey, D.W., Cudney, B.E., Nelson, R.D., Lee, R. P., 2000. Black henbane, Weeds of the West. Western Society of Weed Science, in cooperation with the Western United States Land Grant Universities Cooperative Extension Services and the University of Wyoming, pp. 558-559.

Yang, D.Z., Zhang, Z.Y., Lu, A.M., Kun, S., Liu, J.Q., 2002. Floral organogenesis and development of two taxa in tribe Hyoscyameae (Solanaceae)-Przewalskia tangutica and Hyoscyamus niger. Acta Bot. Sin. 44(8), 889-894.