Phytochemical study and anti-inflammatory activity of the roots of Mangifera indica L. in lipopolysaccharide (LPS)-stimulated peritoneal macrophages

Document Type: Original Article


1 Department of Chemistry, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon

2 Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain

3 Department of Animal Biology, Faculty of Sciences, P.O. Box 67, University of Dschang, Cameroon

4 Department of Animal Biology and Physiology, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon


This study deals with the identification of secondary metabolites of methanol extract of the roots of Mangifera indica L and the assessment of plant in vitro anti-inflammatory activity. High-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) using the negative ion mode was performed to establish the chromatographic fingerprint and identify various chemical components of the plant extract. The anti-inflammatory effect of the MeOH extract (3, 30 and 300 μg/mL) was assessed through cell viability and nitric oxide (NO) production on non-stimulated and LPS-stimulated peritoneal macrophages. Phytochemical analysis indicated the presence of a number of phenolic compounds where galloyl derivatives, mangiferin and its derivatives were the major constituents. The methanol extract exhibited significant concentration-dependent inhibitory effect on NO production, both on stimulated and non-stimulated macrophages. The concentration 300 μg/mL showed significant cell toxicity. The methanol extract of Mangifera indica is rich in phenolic compounds and possesses potent in vitro anti-inflammatory activity, but its higher concentrations are cytotoxic.

Graphical Abstract

Phytochemical study and anti-inflammatory activity of the roots of Mangifera indica L. in lipopolysaccharide (LPS)-stimulated peritoneal macrophages


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