Genetic variability, D2 analysis and characters association among quantitative and qualitative traits of spearmint (Mentha spicata L.)

Document Type: Original Article


1 Central Institute of Medicinal and Aromatic Plants (CSIR- CIMAP), Research Centre, Pantnagar, P.O. - Dairy Farm Nagla, Udham Singh Nagar, Uttarakhand-263149, India

2 CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226015, Uttar Pradesh, India


An investigation was carried out to assess the genetic variability, genetic divergence and association of pheno-morphic and agronomic traits along with major chemical constituents of essential oil in 41 accessions of spearmint (Mentha spicata L.). Highly significant differences were noted for all 10 economic traits indicating the existence of considerable genetic variability among 41 accessions. High heritability (h2) and high genetic advance was noted for herb yield (120.64 %). On the basis of Mahalnobis D2 values, all 41 spearmint accessions were grouped into six diverse clusters. The cluster-I was largest group which consist of 20 accessions. A significant and positive correlation was observed for plant height with herb yield (0.58 * *; ** = significant at 1% probability level). The herb yield showed a highest direct effect (0.194) for oil yield. According to results, a significant genetic variability was present among 41 accessions, simple selection can be employed to improve essential oil content. More importance should be given to plant height, leaf length, leaf width and herb yield during selection to improve essential oil yield in spearmint.

Graphical Abstract

Genetic variability, D2 analysis and characters association among quantitative and qualitative traits of spearmint (Mentha spicata L.)


Main Subjects

Ahmadzadeh, A.R., Alizadeh, B., Shahryar H.A., Narimani, R.M., 2012. Path analysis of the relationships between grain yield and some morphological characters in spring safflower (Carthamus tinctorius L.) under normal irrigation and drought stress condition. J. Med. Plants Res. 6(7), 1268-1270.

Almeida, E.T.D.C., de Souza, G.T., de Sousa Guedes, J.P., Barbosa, I.M., de Sousa, C.P., Castellano, L.R.C., Magnani, M., de Souza, E.L., 2019. Mentha piperita L. essential oil inactivates spoilage yeasts in fruit juices through the perturbation of different physiological functions in yeast cells. Food Microbiol. 82, 20-29.

Atal, C.K., Kapur, B.M., 1982. Cultivation and utilization of aromatic plants. CSIR, Jammu Tawi, India. p. 763.

Chopra, R.N., Nayar, S.L., Chopra, I.C., 1950. Glossary of Indian medicinal plants, CSIR, New Delhi, India, pp. 165-166.

 De Carvalho, C.C.C.R., Da Fonseca, M.M.R., 2006. Food Chem. 95(3), 413-422.

 Dewey, D.I., Lu, K.H., 1959. A correlation and path-coefficient analysis of components of crested wheat grass seed production. Agron. J. 51, 515-518.

Gravois, K.A., Mcnew, R.W., 1993. Genetic relationships among and selection for rice yield and yield components. Crop Sci. 33, 249-252.

Guljar I.D., Patil, S., 2016. Character association and path analysis in safflower germplasm (Carthamus tinctorius L.). Res. J. Agric. Sci. 7(1), 155-157.

Gupta, A.K., Mishra, R., Singh, A.K., Srivastava, A., Lal, R.K., 2017. Genetic variability and correlations of essential oil yield with agro-economic traits in Mentha species and identification of promising cultivars. Ind. Crops Prod. 95, 726-732.

 Hossein, M.N., Mohammad, B.R., Kamkar, J., 2001. Path analysis of the essential oil related characters in Mentha spp. Flav. Fragr. J. 16, 340-343.

 Jirovetz, L., Buchbauer, G., Shabi, M., Ngassoum, M.B., 2002. Comparative investigation of essential oil and volatiles of spearmint. Perfum. Flav. 27, 16-22.

 Kang, M.S., Miller, J.D., Tai, P.Y.P., 1983. Genetic and phenotypic path analyses and heritability in sugarcane. Crop Sci. 23, 643-647.

 Kukreja, A.K., Dhawan, O.P., Ahuja, P.S., Sharma, S., Mathur, A.K., 1992. Genetic improvements of mints: On the qualitative traits of essential oil of in vitro derived clones of Japanese mint (Mentha arvensis var Piperascens Holmes). J. Essent. Oil Res. 4, 623-629.

 Kumar, B., Mali, H., Gupta, E., 2014. Genetic variability, character association, and path analysis for economic traits in menthofuran rich half-sib seed progeny of (Mentha piperita L.) BioMed Res. Int. 7.
Lal, R.K., Sharma, J.R., Singh, N., Misra, H.O., Naqvi, A., 2001. Genetic association sand diversity in the genetic resources of curry neem (Murraya koenigii). J. Med. Aromat. Plant Sci. 216-221.

 Lee, P., Fred, B., 1998. Spearmint: In a Handbook for Farmers and Investors. Rural Industries Research & Development Corporation, Australian Government.

Mahalanobis, P.C., 1936. On the Generalized Distance in Statistics. Proceedings of the National Institute of Sciences of India 2, 49-55.

Mary, S.S., Gopalan, A., 2006. Dissection of genetic attributes yield traits of fodder cowpea in F3 and F4. J. Appl. Sci. Res. 2(6), 805-808.

 Mkaddem, M., Bouajila, J., Ennajar, M., Lebrihi, A., Mathieu, F., Romdhane, M., 2009. Chemical composition and anti-microbial and anti-oxidant activities of (Mentha longifolia L. and viridis) essential oils. J. Food. Sci. 74,358-363.

Nazem, V., Sabzalian, M.R., Saeidi, G., Rahimmalek, M., 2019. Essential oil yield and composition and secondary metabolites in self- and open-pollinated populations of mint (Mentha spp.). Ind. Crops Prod. 130, 332-340.

Nemati Lafmejani, Z., Jafari, A.A., Moradi, P., Ladan Moghadam, A., 2018. Impact of foliar application of iron-chelate and iron nano particles on some morpho-physiological traits and rssential oil composition of peppermint (Mentha piperita L.). J. Essent. Oil-Bear. Plants 21, 1374-1384.

 Panse, V.G., Sukhatme, P.V., 1976. Statistical methods for agricultural workers. ICAR, New Delhi, pp. 361.

Ramanujam, S., Tiwari, A.S., Mehra, R.B., 1974. Genetic divergence and hybrid performance in mung bean. Theor. Appl. Genet.45, 211-214.

Rao, C.R., 1952. Advanced Statistical Methods in Biometric Research. John Wiley and Sons, New York, pp. 1-104.

 Reynolds, E.E. (Ed). 1982. Martindale: The Extra Pharmacopoeia, 28th Edition. The Pharmaceutical Press, London. P. 683.

Shukla, S., Yadav, H.K., Rastogi, A., Mishra, B.K., Singh, S.P., 2010. Diversity in relation to breeding for specific alkaloids in opium poppy (Papaver somniferum L.). J. Genet. Plant Breed. 46, 164-169.

Singh, A.K., Singh, S.B., Singh, S.M., 1996. Genetic divergence in scented and fine genotype of rice. Ann. Agric. Res. 17, 163-166.

Singh, H.P., Patra, N.K., Singh, S.P., 1995. D2 and canonical analysis in relation to the essential oil content potential in scented geranium (Pelargonium graveolens). Curr. Res. Med. Arom. Plants 332-337.

Singh, R.K., Chaudhary, B.D., 1979. Variance and covariance analysis:Biometrical methods in quantitative genetic analysis. Kalyani Publisher, New Delhi (India), p. 57.

 Singh, R.K., Chaudhary, B.D., 1985. Biometrics in quantitative genetics analysis. Kalayani Publishers, New Delhi, pp. 39-79.

Singh, S., Rizivi, H., Lal, R.K., Sarkar, S., Gupta, P., Rai, S.K., 2014. Genetic variability and character associations among economic traits in fennel (Foeniculum vulgare Miller). J. Essent. Oil-Bear. Plants 17(6), 1367-1372.

Soilhi, Z., Rhimi, A., Heuskin, S., Fauconnier, M.L., Mekki, M., 2019. Essential oil chemical diversity of Tunisian Mentha spp. collection. Ind. Crops Prod. 131, 330-340.

Tahir, K., Razi, M.J., 2016. Genetic variability and heritability in F4 populations of Brassica napus L. Sarhad J. Agric. 32, 96-103.

Uemura, M., Hata, G., Toda, T., Weine, F.S., 1997. Effectiveness of eucalyptol and d-limonene as gutta-percha solvents. J. Endod. 23, 739-741.

Yusuf, M., Chowdhury, J.U., Wahab, M.A., Begum, J., 1994. Medicinal Plants of Bangladesh. Bangladesh Council of Scientific and Industrial Research. Chittagong-4220, Bangladesh, p.164.