Antioxidant activity of Allanblackia floribunda trunk bark ethanol extract on alcohol-induced hypertensive rats

Document Type: Original Article

Authors

1 Department of Animal Biology and Physiology, Laboratory of Animal Physiology, University of Yaounde I, P.O. Box 812 Yaounde, Cameroon

2 Department of Biochemisty, Laboratory of phytochemistry, University of Yaounde I, P.O. Box 812 Yaounde, Cameroon

Abstract

Oxidative stress is involved in many chronic diseases. The ethanol extract of Allanblackia floribunda (A. floribunda) was investigated for the antioxidant and antihypertensive activities on alcohol hypertensive rat’s model. The free radical scavenging and antioxidant activities of plant extracts were evaluated in vitro by DPPH and ABTS assays, the ferric reducing antioxidant power assay and chelating ability on ferrous ions. Alcohol-induced hypertensive rats (AHR) were obtained by oral administration of ethanol (3 g/kg/day during 8 weeks) followed by 4 weeks of concomitant treatment with A. floribunda (100 and 200 mg/kg) and ethanol. Hemodynamic parameters were evaluated using the direct cannulation method. The effects of the extract on lipid profile, kidney and liver functions as well as oxidative stress markers were evaluated using colorimetric method. A. floribunda significantly reduced the mean blood pressure. The plant extract protected against the deterioration in lipid profile, reduced kidney and liver impairment, malondialdehyde (MDA) and increased the reduced glutathione (GSH) concentrations in organs. The total phenolic content of A. floribunda extract was found to be 76772.5 μg equivalent catechin/g. Antioxidant potential of the extract was higher with DPPH assay as compared to ABTS assay. These results suggest that the protective effect of the ethanol extract of A. floribunda in alcohol-induced hypertension was in part due to its antioxidant properties.

Graphical Abstract

Antioxidant activity of Allanblackia floribunda trunk bark ethanol extract on alcohol-induced hypertensive rats

Keywords


Aikawa, M., Sugiyama, S., Hill, C.C., Voglic, S.J., Rabkin, E., Fukumoto, Y., Schoen, F.J., Witztum, J.L., Libby, P. 2008. Lipid lowering reduces oxidative stress and endothelial cell activation in rabit atheroma. Circulation 106, 1390-1396.

Apak, R., Güçlü, K., Ozyürek, M., Bektaşoğlu, B., Bener, M., 2010. Cupric ion reducing antioxidant capacity assay for antioxidants in human serum and for hydroxyl radical scavengers. Methods Mol. Biol. 594, 215-239.

Kasrati, A., Alaoui Jamali, C., Abbad, A., 2017. Antioxidant properties of various extract from selected wild Moroccan aromatic and medicinal species. Trends Phytochem. Res. 1(4), 175-182.

Banerjee, J., Das, A., Sinha, M., Saha, S., 2018. Biological efficacy of medicinal plant extracts in preventing oxidative damage. Oxid. Med. Cell Longev. ID 7904349.

Bilanda, D.C., Dimo, T., Djomeni, D.D.P., Bella, T.M.N., Aboubakar, O.B.F., Nguelefack, B.T., Tan, V.P., Kamtchouing, P., 2010. Antihypertensive and antioxidant effects of Allanblackia floribunda Oliv. (Clusiaceae) aqueous in alcohol- and sucrose-induced hypertensive rats. J. Ethnopharmacol. 128, 634-640.

Bilanda, D.C., Dzeufiet, D.P.D., Bopda, A.M., Kamtchuoing, P., Dimo, T., 2018. Allablanckia floribunda hypotensive activity on ethanol induced hypertension in rat. J. Phytopharmacol. 7(2), 146-151.

Boudjeko, T., Kanemoto, N.J.E., Woguia, A.L., Njintang, Y.N., 2013. Partial characterization, antioxidative properties and hypolipidemic efects of oil seed cake of Allanblackia floribunda and Jatropha curcas. BMC Complement. Altern. Med. 13, 352- 362.

Boudjeko, T., Megnekou, R., Woguia, A., Kegne, F.M., Ngomoyogoli, J.E., Tchapoum, C.D., Koum, O., 2015. Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves. BMC Res. Notes. 8, 759-768.

Conforti, F., Loizzo, M.R., Statti, G.A., Menichini, F., 2005. Comparative radical scavenging and antidiabetic activities of methanolic extract and fractions from Achillea ligustica. All. Biol. Pharm. Bull. 28(9), 1791-1794.

Elks, C.M., Reed, S.D., Mariappan, N., Shukitt-Hale, B., Joseph, J.A., Ingram, D.K., Francis, J.A., 2011. Blueberry-enriched diet attenuates nephropathy in a rat model of hypertension via reduction in oxidative stress. PLOS ONE 6(9), 1-10.

 Fadilah, F., Arsianti, A., Yanuar, A., Andrajati, R., Paramita, R.I., Purwaningsih, E.H., 2018. Structure activity relationship analysis of antioxidant activity of simple benzene carboxylic acids group based on multiple linear regression. Orient. J. Chem. 34(5), 1214-1224.

Husain, K., Ansari, R., Ferder, L., 2014. Alcohol-induced hypertension: Mechanism and prevention. World. J. Cardiol. 6, 245-252.

Kada, A.S., Miegueu, P., Bilanda, D.C., Nya Ngassam, F.M., Watco, P., Dzeufiet, D.P.D., Kamtchouing, P., 2013. Ejaculatory activities of Allanblackia floribunda stem back in spinal male rats. Pharm. Biol. 51(8), 1014-1020.

Kalantari, H., Foruozandeh, H., Khodayar, M.J., Siahpoosh, A., Saki, N., Kheradmand, P., 2018. Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice. JTCM 8(1), 120-127.

Karuna, D.S., Dey, P., Das, S., Kundu, A., Tejendra, B., 2018. In vitro antioxidant activities of root extract of Asparagus racemosus Linn. JTCM 8(1), 60-65.

Kukongviriyapan, U., Luangaram, S., Leekhaosoong, K., Kukongviriyapan, V., Preeprame, S., 2007. Antioxidant and vascular protective activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biol. Pharm. Bull. 30, 661-666.

Lin, H-Y., Chang, T-C., Chang, S-T., 2018. A review of antioxidant and pharmacological properties of phenolic compounds in Acacia confuse. JTCM 8, 443-450.

Mediesse-Kengne, F., Woguia, AL., Fogue, P., Atogho-Tiedeu, B., Simo, G., Boudjeko, T., 2014. Antioxidant properties of cell wall polysaccharides of Stevia rebaudiana leaves. J. Cost Life Med. 2(12), 962-969.

Misra, H., Fridovish, I., 1972. Determination of the level of superoxide dismutase in whole blood. New Haven, Yale University Press, pp. 101-109.

 Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities - A review. Ind. Crops Prod. 129, 350-394.

Montezano, A.C., Dulak-Lis, M., Tsiropoulou, S., Harvey, A., Briones, A.M., Touyz, R.M., 2015. Oxidative stress and human hypertension, vascular mechanisms, biomarkers, and novel therapies. Can. J. Cardiol. 31(5), 631-641.

 Odebiyi, A., Sofowora, A.E., 1999. Phytochemical screenings of Nigerian medicinal plants part. Lyodia. A.E. 44, 234-246.

Sarker, S.D., Nahar, L., 2018. Phytochemicals and phyto-extracts in cosmetics. Trends Phytochem. Res. 2(4), 185-186.

Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am. J. Enol. Viticult. 16, 144-158.

Small, H.Y., Migliarino, S., Czesnikiewicz-Guzik, M., Guzik, T.J., 2018. Hypertension: Focus on autoimmunity and oxidative stress. Free Radic. Biol. Med. 125, 104-115.

Sorriento, D., De Luca, N., Trimarco, B., Iaccarino, G., 2018. The antioxidant therapy, new insights in the treatment of hypertension. Front. Physiol. 9, 258-265.

Wakayashi, I., Kobaba, W.R., 2002. Effet de l’âge sur le rapport entre le boire et les rapports arthérosclérotiques. Gerontology 48, 151-156.

Wang, M., Li, J., Rangarajan M.., Shao, Y., Lavoie, EJ., Huang, TC., Ho, C.T., 1998. Antioxidative phenolic compounds from Sage (Salvia officinalis). J. Agric. Food Chem. 46, 4869-4873.

Wilbur, K.M., Bernheim, F., Shapiro, O., 1949. Determination of lipid peroxidation. Arch Biochem. 24, 305-310.

Ipek, Y., Ozen, T., Demirtas, I., 2017. Extraction, purification, characterization and antioxidant activities of heat-resistance phenolic compounds from lemon pulp. Trends Phytochem Res. 1(1), 199-206.