Phytochemical analysis and screening of antioxidant, antibacterial and anti-inflammatory activity of essential oil of Premna mucronata Roxb. leaves

Document Type: Original Article


Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India


Premna mucronata Roxb. commonly known as agyon, is a plant of family Lamiaceae. This study dealt with the phytochemical analysis of leaves essential oil of Premna mucronata Roxb. (PMLO) using GC/MS technique and screening of its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH), metal chelating and reducing power assays, anti-inflammatory activity using in-vitro albumin denaturation assay and antibacterial activity using well diffusion method against pathogenic bacterial strains Escherichia coli and Staphylococcus aureus. The phytochemical analysis of PMLO revealed the presence of over 71 compounds. 3-octanone was found to be the major constituent that accounts for 23.6% of the total oil composition. The other major constituents identified were ethyl-hexanol (13.9%), 1-octen-3-ol (9.6%), linalool (5.5%), methyl salicylate (2.9%) and E-caryophyllene (2.9%). It was found that PMLO possessed antioxidant activity in terms of DPPH (IC50=11.18±0.03 µg/mL), metal chelating (IC50=18.82±0.46 µg/mL), and reducing power activities (IC50=21.69±0.02 µg/mL) and in-vitro anti-inflammatory activity was also shown by essential oil with IC50=30.27±0.005 µg/mL. Antibacterial activity of PMLO at higher concentration (1000ppm) showed zone of inhibition of 14.33±0.58mm against E. coli and 14.66±0.58mm against S. aureus. The essential oil of leave P.mucronata contains 3-Octanone, 1-octen-3-ol, ethyl-hexanol, linalool, and have antioxidant, anti-inflammation and antibacterial acitivities.

Graphical Abstract

Phytochemical analysis and screening of antioxidant, antibacterial and anti-inflammatory activity of essential oil of Premna mucronata Roxb. leaves


Main Subjects

Adams, R.P., 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry 4th Ed. Allured Publishing Corporation: Carol Stream, Illinois, USA.

 Ali, A.A., Chhetri, B.K., Dosoky, N.S., Shari, K., Al-Fahad, A.J.A., Wessjohann, L., Setzer W.N., 2017. Antimicrobial, antioxidant, and cytotoxic activities of Ocimum forskolei and Teucrium yemense (Lamiaceae) essential oils. Medicines (Basel) 4(2), 17.

Arunkumar, R., Nair, S.A., Rameshkumar, K.B., Subramoniam, A., 2014. The essential oil constituents of Zornia diphylla (L.) Pers, and anti-inflammatory and antimicrobial activities of the oil. Rec. Nat. Prod. 8(4), 385-393.

Balouri, M., Sadiki, M., Ibnsouda, S.K., 2016. Method for in-vitro evaluating antimicrobial activity: A review. J. Pharm. Anal. 6, 71-79.

Blois, M.S., 1958. Antioxidant determinations by the use of a stable free radical. Nature 181(4617), 1199-1200. Bot. Rev. 73, 67-182.

Chandra, S., Chatterjee, P., Dey, P., Bhattacharya, S., 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac. J. Trop. Biomed. S178-S180.

Dianita, R., Jantan, I., 2017. Ethnomedicinal uses, phytochemistry and pharmacological aspects of the genus Premna: a review. Pharm. Biol. 55(1), 1715-1739.

Dorman, H.J.D., Deans, S.G., 2008. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88(2), 308-316.

 Gertsch, J.,  Leonti, M.,  Raduner, S.,  Racz, I.,  Chen, J.Z., Xie, X.Q.,  Altmann, K.H., Karsak, M., Zimmer, A., 2008. Beta-caryophyllene is a dietary cannabinoid. Proceedings of the National Academy of Sciences of the United States of America, 105(26), pp. 9099-9104.

Harley, R.M., Atkins, S., Budantsev, A.L., Cantino, P.D., Conn, B.J., Grayer, R., Harley, M.M., de Kok, R., Krestovskaja, T., Morales, R., Paton, A.J., 2004. Labiatae. Editors: Kubitzki, K., Kadereit, J.W., The Families and Genera of Vascular Plants. Flowering plants, Dicotyledons: Lamiales (except Acanthaceae including Avicenniaceae) Berlin, Vol. 7, pp. 167-275.

Hart, P.H., Brand, C., Carson, C.F., Riley, T.V., Prager, R.H., Finlay-Jones, J.J., 2000. Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes. Inflamm. Res. 49(11), 619-626.

Held, S., Schieberle, P., Somoza, V., 2007. Characterization of alpha-terpineol as an anti-inflammatory component of orange juice by in vitro studies using oral buccal cells. J. Agric. Food Chem. 55(20), 8040-8046.

Herman, A., Tambor, K., Herman, A., 2016. Linalool affects the antimicrobial efficacy of essential oils. Curr. Microb. 72(2), 165-172.

Javed, M.S., Kumar, P., Kumar, R., Tiwari A.K., Bisht, K.S., 2016. In vitro antifungal, antibacterial and antioxidant activity of essential oil from the aerial parts of Agrimonia aitchisonii schonbeck temesy from Himalayan region. World J. Pharm. Res. 5(3), 1747-1763.

Jena, A.K., Karan, M., Vasisht, K., 2017. Plant parts substitution based approach as a viable conservation strategy for medicinal plants: A case study of Premna latifolia Roxb. J-AIM,8(2017), 68-72.

Krishnamoorthi, R., Bai, V.R., 2015. Phytochemical analysis and antioxidant property of Premna latifolia. Open Sci. J. Pharm. Pharmacol. 3(2), 6-9.

Kumar, A., Tamta, M.L., Negi, N., Chandrasekharb, K., Negi, D.S., 2011. Phytochemical investigation and antifeedant activity of Premna latifolia leaves. Nat. Prod. Res. 25(18), 1680-1686.

Kumari, S.P.H., Caithra, R.P., Nishteswar, K., 2011. A comparative experimental evaluation of anti-inflammatory activity of Premna obtusifolia Linn and Premna latifolia Roxb leaves in Charles foster rats. Anc. Sci. Life 31, 58-61.

Kunwar, G., Prakash, O., Chandra, M., Pant, A.K., 2013. Chemical composition, antifungal and antioxidant activities of Perilla frutescens (L.) syn. P. ocimoides L. collected from different regions of Indian Himalaya. Asian J. Trad. Med. 2013, 8, 88-98.

Liu, H., Qiu, N., Ding, H., Yao, R., 2008. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Res. Int. 41(4), 363-370.

Lu, M., Yuan, B., Zeng, M., Chen, J. 2011. Antioxidant capacity and major phenolic compounds of spices commonly consumed in China. Food Res. Int. 44(2), 530-536.

Mahire, N.B., Tote, M.V., Jain, A.P.,  Undale, V.R., Bhosle, A.V., 2009. Anti-inflammatory effect of Premna latifolia leaves. Pharmacologyonline 3(2009), 929-937.

Mohammadhosseini, M., 2017. Essential oils extracted using microwave-assisted hydrodistillation from aerial parts of eleven Artemisia species: Chemical compositions and diversities in different geographical regions of Iran. Rec. Nat. Prod. 11(2), 114-129.

Mohammadhosseini, M., 2017. The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Ind. Crop Prod. 105, 164-192.

Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities - A review. Ind. Crop Prod. 129, 350-394.

Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.

Montanari, R.M., Barbosa, L.C.A., Demuner, A.J., Silva, C.J., Carvalho, L.S., Andrade, N.J. 2011. Chemical composition and antibacterial activity of essential oils from Verbenaceae species: alternative sources of (E)-caryophyllene and germacrene-D. Quim. Nova. 34(9), 1550-1555.

Munir, A.A., 1984. A taxonomic revision of the genus Premna L. (Verbenaceae) in Australia. Adelaide Bot. Gard. 7, 1-44.

Olmstead, R., 2010. A sypnotical classification of the Lamiales. Version 2.2 [updated 2016 July 26; cited 2015 Oct 20. Available from class.v.2.2 0.pdf

Olmstead, R., 2012. A sypnotical classification of the Lamiales. Version 2.6.2. [updated 2016 April 12]. Available from:

Oloyede, G.K., 2016. Toxicity, antimicrobial and antioxidant activities of methyl salicylate dominated essential oils of Laportea aestuans (Gaud). Arab. J. Chem. 9, S840-S845.

Parki, A., Chaubey, P., Prakash, O., Kumar, R., Pant, A.K., 2017. Seasonal variation in essential oil compositions and antioxidant properties of Acorus calamus L. accessions. Medicines 4(4), 81.

Patel, N.G., Patel, K.G., Patel, K.V., Gandhi, T.R., 2015. Myocardial salvaging effect of Premna mucronata Roxb. (Verbenaceae) on isoproterenol induced myocardial necrosis in rats. Der Pharm. Lett. 7(7), 137-147.

Peana, A.T., D'Aquila, P.S., Panin, F., Serra, G., Pippia, P., Moretti, M.D.L., 2002. Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils. Phytomedicine 9(8), 721-726.

Ram, A.J., Bhakshu, L.M., Venkata Raju, R.R., 2004. In vitro antimicrobial activity of certain medicinal plants from Eastern Ghats, India, used for skin diseases. J. Ethnopharmacol.90, 353-357.

Rekha, K., Richa, P.K., Babu, S., Rao, M., 2015. A phytochemistry of the genus Premna: a review. Int. J. Chem. Pharm. Sci. 4(3), 317-25.

Renjana, P.K., John, E.T., 2013. Larvicidal activities of the leaf extracts and essential oil of Premna latifolia Roxb. (Verbenaceae) against Aedes albopictus Skuse (Diptera: Culicidae). J. App. Pharm. Sci. 3 (06), 101-105.

Savsani, H., Shah, H., Patel, K., Gandhi, T., 2014. Cardioprotective effect of flavonoids rich fraction of Premna mucronata on isoproterenol-induced myocardial infarction in wistar rats. Int. J. Phytopharmacol. 5(2), 95-108.

Seol, G.H., Kang, P., Lee, H.S., Seol, G.H., 2016. Antioxidant activity of linalool in patients with carpal tunnel syndrome. BMC Neurol. 16(1), 17.

Singh, G., Marimuthu, P., Murali, H.S., Bawa, A.S., 2005. Antioxidative and antibacterial potentials of essential oils and extracts isolated from various spice materials. J. Food Saftey 25(2), 130-145.

Suresh, G., Babu, K.S., Rao, V.R.S., Rao, M.S.A., Nayak, V.L., Ramakrishna, S., 2011. Novel cytotoxic icetexane diterpenes from Premna latifolia Roxb. Tetrahedron Lett. 52(12), 1273-1276.

Shunmugaperumal, T., Kaur, V., 2016. In vitro anti-inflammatory and antimicrobial activities of azithromycin after loaded in chitosan- and tween 20-based oil-in-water macroemulsion for acne management. J. Am. Assoc. Pharm. Sci. 17(3), 700-709.

Subedi, L., Timalsena, S., Duwadi, P., Thapa, R., Paudel, A., Parajuli, K., 2014. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J. Trad. Chinese Med.34(5), 584-590.

The Plant List, 2013. Version 1.1. Published on the internet; accessed on 08.06.2017.

Venditti, A., Frezza, C., Serafini, I., Pulone, S., Scardelletti, G., Sciubba, F., Bianco, A., Serafini, M., 2018. Chemical profiling of the fruits of Styrax officinalis L. from Monti Lucretili (Latium region, Central Italy): Chemotaxonomy and nutraceutical potential. Trends Phytochem. Res. 2(1), 1-12.

Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2018. Bioactive essential oils from the Cameroonian rain forest: A review - Part I. Trends Phytochem. Res. 2(4), 187-234.

WCSP, 2018. World Checking of Selected Plant Families. Facilitated by the Royal Botanical Garden, Kew, org/ Retrived 29 May 2018.

Williams, L.A.D., Connar, A.O., Latore, L., Dennis, O., Ringer, S., Whittaker, J.A., Conrad, J., Vogler, B., Rosner, H., Kraus, W., 2008. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (Immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process.West Indian Med. J. 57(4), 327.

Xiong, C., Li, Q., Li, S., Chen, C., Chen, Z., Huang, W., 2017. In vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi. J. Oleo Sci. 66(9) 1041-1049


Yen, G.C., Duh, P.D., Tsai, C.L., 1993. Relationship between antioxidant activity and maturity of peanut hulls. J. Agri. Food Chem. 41(1), 67-70.

Zhang, D., Liu, R., Sun, L., Huang, C., Wang, C., Zhang, D.M., Zhang, T.T., Du, G.H., 2011. Anti-inflammatory activity of methyl salicylate glycosides isolated from Gaultheria yunnanensis (Franch.) Rehder. Molecules 16(5), 3875-3884.

Zhang, Z.M., Wu, W.W., Li, G.K., 2008. A GC-MS study of the volatile organic composition of straw and oyster mushrooms during maturity and its relation to antioxidant activity. J. Chrom. Sci. 46(8), 690-696.