Document Type : Original Article


1 Laboratory of Organic Chemistry and Natural Substances (LCOSN), Félix Houphouet-Boigny University, 22 BP 582 Abidjan 22, Cote d’Ivoire

2 Laboratory of Industrial Processes, Synthesis, Environment and New Energies, National Polytechnic Institute Felix Houphouët-Boigny, BP 1093 Yamoussoukro, Cote d’Ivoire

3 Unit of Antibiotics, Natural Substances and Surveillance of the Resistance of Microorganisms to Anti-Infectives (ASSURMI) of the Department of Bacteriology of the Institut Pasteur Cote d’Ivoire (IPCI), 01 BP 490 Abidjan 01, Cote d’Ivoire


Santaloïdes afzelii (R.Br. ex Planch.) Schellenb (Connaraceae) is used in Africa as an analgesic, aphrodisiac and in the treatment of stomachaches, muscular pains and in medico magic rites. The present study aims to identify the bioactive organic compounds from the bark and seeds of Santaloïdes afzelii collected in Korhogo in the north of Côte d’Ivoire. Microbiological testing againts Staphylococcus aureus of the hydro ethanolic crude extracts of the bark and seeds showed low activities compared to the activities of oxacillin and cefoxitin. The chemical investigations of these extracts led to the identification of gallic acid, epi-catechin and dimers of procyanidin.

Graphical Abstract

Isolation and identification of bioactives organics compounds from the bark and seeds of Santaloïdes afzelii (Connaraceae)


Arbonnier, M., 2009. Trees, Shrubs and Lianas of West African Dry Zones, F-78026 Versailles cedex. Edition ISBN-Quae-978-2-7592-0313-0, Service of the Scientific Publication, pp. 279-280.
Baumer, M., 1995. Arbres, Arbustes et Arbrisseaux Nourriciers en Afrique Occidentale. ENDA Editions, Dakar, pp. 260-266.
Bouquet, A., Debray, M., 1974. Plantes Médicinales de la Côte d’Ivoire, Travaux et Document de l’O.R.S.T.O.M. n°32, pp.75-76
Shen, C.C., Chang, Y.S., Ho, L.K., 1993. Nuclear magnetic resonance studies of 5,7-dihydroxyflavonoids. Phytochemistry 34(3), 843-845.
Chritophe, W., 2006. Medicinal Plants of the Aisa-pacific, Drugs for the Future. University of Malaya, Malaysia, World Scientific Publishing, pp. 240-246.
Foo, L.Y., Lu, Y., 1999. Isolation and identification of procyanidins in apple pomace. Food Chem. 64(4), 511-518.
Golly, K.J., Siaka, S., Guessennd, N., Soro, Y., Djama, A.J., Dosso, M., 2012. Phytochemical assessment and antimicrobial activity of leaves extract of Vernonia colorata (Wild.) Drake on resistant germs of Staphylococcus aureus and Pseudomonas aeruginosa. J. Chem. Pharm. Res. 4(5), 2490-2494.
Guy, R., 1954. Notes sur la Flore de l’ouest-africain. Institut français d’Afrique Noire, Tome XVI, N°2, Série A, pp. 321-369.
Ambé, G.A., 2001. Les fruits sauvages comestibles en savanes guineennes de Côte d’Ivoire: état de la connaissance par une population locale, les malinkés. Biotechnol. Agron. Soc. Environ. 5(1), 43-48.
Kalegari, M., Miguel, M.D., Dias, J.D.F.G., Lordello, A.L.L., Lima, C.P.D., Miyazaki, C.M.S., Zanin, S.M.W., Verdam, M.C.D.S., Miguel, O.G., 2011. Phytochemical constituents and preliminary toxicity evaluation of leaves from Rourea induta Planch. (Connaraceae). Brazilian J. Pharm. Sci. 47(3), 635-642
Korul’kina, L.M., Shul’ts, E.E., Zhusupova, G.E., Abilov, Z.A., Erzhanov, K.B., Chaudri, M.I., 2004. Biologically active compounds from Limonium Gmelinii and L. popovii. Chem. Nat. Compd. 40(5), 465-471.
Lin, Y.P., Chen, T.Y., Tseng, H.W., Lee, M.H., Chen, S.T., 2009. Neural cell protective compounds isolated from Phoenix hanceana var. formosana. Phytochemistry 70(9), 1173-1181
Lokvam, J., Coley, P.D., Kursar, T.A., 2004. Cinnamoyl glucosides of catechin and dimeric procyanidins from young leaves of Inga umbellifera (Fabaceae). Phytochemistry 65(3), 351-358.
Matti, V., 2014. Flavonoids and other phenolic compounds: characterization and interactions with lepidopteran and sawfly larvae. University of Turku, pp.12-22
Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities. J. Ethnopharmacol. 199, 257-315.
Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities, Ind. Corp Prod. 129, 350-394.
Nunes, H., Miguel, M.G., 2017. Rosa damascena essential oils: a brief review about chemical composition and biological properties. Trends Phytochem. Res. 1(3), 111-128.
Oliveira, P.V.D., Lemos, R.P., Conserva, L.M., 2012.Chemical constituents of Rourea doniana.Rev. Bras. Farmacogn.22(2), 451-454.
OMS, 2000. Principes méthodologiques généraux pour la recherche et l'évaluation relatives à la médecine traditionnelle, pp.87-88
Pretsch, E., Bühlman, P., Badertscher, M., 2009. Structure Determination of Organic Compounds, Tables of Spectral Data, 4th Revised and Enlarged Edition, Springer,pp. 408-409.
Soong, Y.Y., Barlow, P.J., 2006. Quantification of gallic acid and ellagic acid from longan (Dimocarpus longan Lour.) seed and mango (Mangifera indica L.) kernel and their effects on antioxidant activity. Food Chem. 97(3), 524-530.
Soro, Y., Kassi, A.B.B., Bamba, F., Sorho, S., Toure, S.A., Coustard, J.M., 2012. Flavonoids and gallic acid from leaves of Santaloides afzelii (Connaraceae). Rasayan J. Chem. 5(3), 332-337.
Stark, T., Bareuther, S., Hofmann, T., 2005. Sensory-guided decomposition of roasted Cocoa Nibs (Theobroma cacao) and structure determination of taste-active polyphenols. J. Agric. Food Chem. 53(13), 5407-5418.
Sun, J., Liang, F., Bin, Y., Li, P., Duan, C., 2007.Screening non-colored phenolics in red wines using liquid chromatography/ultraviolet and mass spectrometry/mass spectrometry libraries. Molecules12(3), 679-693.
Svedström, U., Vuorela, H., Kostiainen, R., Tuominen, J., Kokkonen, J., Rauha, J.P., Laakso, I., Hiltunen, R., 2002. Isolation and identification of oligomeric procyanidins from Crataegus leaves and flowers. Phytochemistry. 60(8), 821-825.
Tarascou, I., 2005. Synthèse et Caractérisation de Procyanidines Oligomères pour l’identification de Tanins du Raisin et du vin. Universite bordeaux, pp. 60-70.
Venditti, A., Bianco, A., 2018. Secondary metabolites of Hypericum richeri Vill. collected in Central Italy: chemotaxonomy and ethnomedicinal relevance. Trends Phytochem. Res. 2(3), 155-162.
Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2019. Bioactive essential oils from the Cameroonian rain forest: A review - Part II. Trends Phytochem. Res. 3(1), 3-52.
Wei, S.D., Zhou, H.C., Lin, Y.M., Liao, M.M. and Chai, W.M., 2010. MALDI-TOF MS analysis of condensed tannins with potent antioxidant activity from the leaf, stem bark and root Bark of Acacia confusa. Molecules 15(6), 4369-4381.
Zakaria, Z.A., Zaiton, H., Henie, E.F.P., Mat Jais, A.M., Kasthuri, D., 2006. The in vitro antibacterial activity of Corchorus olitorius and Muntingia calabura extracts. J. Pharm. Toxicol. 1 (2), 108-114.
Zhang, S.J., Lin, Y.M., Zhou, H.C., Wei, S.D., Lin, G.H., Ye, G.F., 2010. Antioxidant tannins from stem bark and fine root of Casuarina equisetifolia. Molecules15(8), 5658-5670.