Document Type : Review article


1 Department of Dravyaguna Vignana, Institute of Indigenous Medicine, University of Colombo, Sri Lanka

2 Department of Pharmacology, Faculty of Medicine, University of Colombo, Sri Lanka

3 Research and Development Complex, Industrial Technology Institute, Malabe, Sri Lanka


Phyllanthus niruri Linn belongs to plant family Euphorbiaceae and is found in tropical and subtropical countries of the world including some regions of Sri Lanka and India. The extract of P. niruri Linn is the most commonly used medicine in Ayurveda system of medicine and has especially been recommended for the treatment of bronchitis, anaemia, skin diseases, asthma, cough, liver, kidney and urinary tract disorders. In the present study, an attempt was done to document the scientific investigations on phytochemicals and pharmacological activities of P. niruri Linn. According to the findings of P. niruri Linn, it shows potent pharmacological actions on hepatitis B virus, liver carcinoma, kidney stones and HIV. The available research suggested that P. niruri Linn has many beneficial health effects. However, human based data are sparse and conducting clinical trials will be necessary to prove more public health implications. These findings may be considered as clues to the development of novel pharmaceutical preparations from P. niruri Linn in future.

Graphical Abstract

A mini review on therapeutic potentials of Phyllanthus niruri L.


Ajibade, V.A., Ajenifuja, O.A., Akinruli, F.T., Ajayi, F.A., Famurewa, O., 2018. Antifungal efficacy of saponin extracted from Phyllanthusniruri. Int. J. Pathol. Res. 1(3), 1-8.
Anupika, S., Kaur, R., Kaur, J., Kaur, S., 2016. In vitro and in vivo evaluation of antileishmanial activity of Phyllanthusniruri (Family: Euphorbiaceae). Int. J. Trop. Dis. Health. 13(1), 1-15.
Barros, M.E., Lima, R., Mercuri, L.P., Matos, J.R., Schor, N., Boim, M.A., 2006. Effect of extract of Phyllanthus niruri on crystal deposition in experimental urolithiasis. Urol. Res. 34(6), 351-7.
Becker, C.G., Hajjar, D.P., Hefton, J.M., 1985. Tobacco constituents are mitogenic for arterial smooth-muscle cells. Am. J. Pathol. 120, 1-5.
Boim, M.A., Heilberg, I.P., Schor, N., 2010. Phyllanthus niruri as a promising alternative treatment for Nephrolithiasis. Int. Braz. J. Urol. 36(6), 657-64.
Damanhouri, Z.A., Ahmad, A.A., 2014. Review on therapeutic potential of Piper nigrum L. (Black Pepper): The king of spices. Med. Aromat. Plants. 3, 3.
De AraújoJúnior, R.F., Soares, L.A.L., De Costa Porto, C.R., Furtado de Aquino, R.G., Guedes, H.G., Petrovick, P.R., 2012. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World J. Gastroenterol. 18(31), 4162-4168.
Dodevic, N.O., Todovovic, N., Novakovic, I.T., Pezo, L.L., Pejin, B., Maras, V., Tesevic, V.V., Pajovic, S.B., 2018. Antioxidant activity of selected polyphenolics in yeast cells: The case study of Montenegrin Merlot wine. Molecules. 23, 1971.
Dnyaneshwar, U., Bawankule, P., Trivedi, A., Pal, K., Shanker, M., 2014. Protective mechanism of lignans from Phyllanthusamarus against galactosamine/lipopolysaccharide-induced hepatitis: An in vivo and in-Silico studies. Curr. Top. Med.Chem. 14, 1045-1055.
Gao, Z., Xu, H., Chen, X., 2003. Antioxidant status and mineral contents in tissues of rutin and baicalin fed rats. Life Sci. 73, 1599-1607.
Geeta, T., Varalakshmi, P., 2001. Anti-inflammatory activity of lupeol and lupeollinoleate in rats. J. Ethnopharmacol. 76, 77-80.
Giribabu, Rao, P.V., Kumar, K.P., Muniandy, S., Rekha, S.S., Salleh, N., 2014. Aqueous extract of Phyllanthusniruri leaves displays in vitro antioxidant activity and prevents the elevation of oxidative stress in the kidney of Streptozotocin-induced diabetic male rats. Evid-Based Complement. Altern. Med.
Gujjeti, R.P., Estari, M., 2015. In vitro cytotoxic and anti-HIV activity of Phyllanthus niruri whole plant extracts. Int. J. Pharma Bio. Sci. 6(2), 487-493.
Gupta, D.R., Ahmed, B.Z., Shoyakugaku, 1984. A new flavone glycoside from Phyllanthusniruri. J. Nat. Prod. 383, 213-215.
Harish, R., Shivanandappa, T., 2006. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chem. 95, 180-185.
Hutomo, S., Putri, D.U., Suryanto, Y.I., Susilowati, H., 2018. Potential immunomodulatory activity of Phyllanthus niruri aqueous extract on macrophage infected with Streptococcus sanguinis. Dent. J.51(3), 124-128.
Jantan, I., Haque, A., Ilangkovan, M., Arshad, L., 2019. An insight into the modulatory effects and mechanisms of actions of Phyllanthus species and their bioactive metabolites on the immune system. Front. Pharmacol. 10(878).
Jose, J., Sudhakaran, S., Kumar, T.M.S., Jayaraman, S., Variyar, E.J., 2014. Study of in vitro immunomodulatory effect of flavonoid isolated from Phyllanthus niruri on human blood lymphocytes and evaluation of its antioxidant potential. Int. J. Pharmacogn Phytochem. Res. 6(2), 284-289.
Kale, K.U., Parag, D., Vivek, C., 2001. Isolation and estimation of antihepatotoxic compound from Phyllanthusniruri. Indian Drugs. 38, 303-306.
Khanna, A.K., Rizvi, F., Chander, R., 2002. Lipid lowering activity of Phyllanthus niruri in hyperlipemic rats. J. Ethnopharmacol. 82, 19-22.
Köse, L.P., Gülçin, I., Gören, A.C., Namiesnik, J., Martinez-Ayala, A.L., Gorinstein, S., 2015. LC-MS/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes. Ind Crop Prod. 74, 712-721.
Lee, N.Y.S., Khooa, W.K.S., Adnana, M.A., Mahalingam, T.P., Fernandez, A.R., Jeevaratnam, K., 2016. The pharmacological potential of Phyllanthusniruri.J. Pharm. Pharmacol. 68,953-969.
Lizuka, T., Nagai, M., 2006. Vaso-relaxant effect of methyl brevifolin carboxylate from the leaves of Phyllanthusniruri. Biol. Pharm. Bull. 29, 177-179.
Malini, M.M., Lenin, M., Varalakshmi, P., 2000. Protective effect of triterpenes on calcium oxalate crystal-induced peroxidative changes in experimental urolithiasis. Pharmacol. Res. 41(4), 413-418.
Manonmani, P., Ramar, M., Geetha, N., Arasu, M.E., Raskin, E., Sowmiya, J.J., 2015. Hepatoprotective activity of aqueous extract of Phyllanthus niruri in CCl4 induced liver toxicity-in vivo study. Res. J. Biotechnol. 10 (9), 11-17.
Micali, S., Sighinolfi, M.C., Celia, A., De Stefani,  S., Grande, M., Cicero, A.F., Bianchi, G., 2006. Can Phyllanthus niruri affect the efficacy of extracorporeal shock wave lithotripsy for renal stones? A randomized, prospective, long-term study. J. Urol. 176(3), 1020-1022.
Mills, J.J., Chari, R.S., Boyer, I.J., Gould, M.N., Jirtle, R.L., 1995. Induction of apoptosisin liver tumors by monoterpene perillyl alcohol. Cancer Res. 55, 979-983.
Mohammadhosseini, M., 2017. The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Ind. Crop Prod. 105, 164-192.
Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.
Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review. Ind. Crop Prod. 129, 350-394.
Mostofa, R., Ahmed, S., Begum, M., Rahman, S., Begum, T., Ahmed, S.U., 2017. Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats. BMC Complement. Altern. Med. 17, 1771-1777.
Naik, A.D., Juvekar, A.R., 2003. Effects of alkaloidal extract of Phyllanthus niruri on HIV Replication. Indian J. Med. Sci. 57(9), 387-393.
Narendra, K., Swathi, J., Sowjanya, K.M., Satya A.K., 2012. Phyllanthusniruri: A review on its ethno botanical, phytochemical and pharmacological profile. J. Pharma. Res. 5(9), 4681-4691.
Ogata, T., Higuchi, H., Mochida, S., Matsumoto, H., Kato, A., Endo, T., Kaji, A., Kaji, H., 1992. HIV-1 reverse transcriptase inhibitor from Phyllanthus niruri. AIDS Res. Hum. Retroviruses. 8(11), 1937-44.
Okoli, C.O., Ibiam, A.F., Ezike, A.C., Akah, P.A., Okoye, T.C., 2010. Evaluation of antidiabetic potentials of Phyllanthus niruri in alloxan diabetic rats. Afr. J. Biotechnol. 9(2), 248-259.
Ooi, K.L., Loh, S.I., Sattar, M.A., Muhammad, T.S.T., Sulaiman, S.F., 2015. Cytotoxic, caspase-3 induction and in vivo hepatoprotective effects of phyllanthin, a major constituent of Phyllanthus niruri. J. Funct. Foods. 14, 236-245.
Penjin, B., Ciric, A., Markovic, J.D., Glamoclija, J., Nikolic, M., Stanimirovic, B., Sokovic, M., 2015. Quercetin potently reduces biofilm formation of the strain Pseudomonas aeruginosa PAO1 in vitro. Curr. Pharm. Biotech. 16(8).
Porto, C.R.C., Soares, L.A.L., Souza, T.P., Petrovick, P.R., Lyra, I.L., Araújo, R.F., 2013. Anti-inflammatory and antinociceptive activities of Phyllanthus niruri spray-dried standardized extract. Rev. Bras. Farmacogn. 23(1), 138-144.
Rusmana, D., Wahyudianingsih, R., Elisabeth, M., Maesaroh, B., Widowati, W., 2017. Antioxidant activity of Phyllanthus niruri extract, rutin and quercetin. Indones. Biomed. J. 9(2), p. 84-90.
Saija, A., Tomaino, A., Trombetta, D., Pellegrino, M.L., Tita, B., Messina, C., Bonina, F.P., Rocco, C., Nicolosi, G., Castelli, F., 2003. In vitro antioxidant and photo protective properties and interaction with model membranes of three new quercetin esters. Eur. J. Pharm. Biopharm. 56, 167-174.
Sarkar, M.K., Sil, P.C., 2007. Hepatocytes are protected by herb Phyllanthus niruri protein isolate against thioacetamide toxicity. Pathophysiology. 14, 113-120.
Sarker, S.D., Nahar, L., 2018. Phytochemicals and phyto-extracts in cosmetics. Trends Phytochem. Res. 2(4), 185-186.
Sathisha, A., Udupa, L., Rathnakar, U.P., Pal, P.G., Acharya, S., Shastry, R., 2009. Anti-inflammatory and analgesic activity of Phyllanthus niruri in rodent models. Indian Drugs. 46(12), 50-53.
Shanbhag, T., Amuthan, A., Shenoy. S., Sudhakar, 2010. Effect of Phyllanthus niruri Linn. on burn wound in rats. Asian Pac. J. Trop. Med. 105-108.
Sharma, P., Parmar, J., Verma, P., Sharma, P., Goyal, P.K., 2009. Anti-tumor activity of Phyllanthusniruri (a medicinal plant) on chemical-induced skin carcinogenesis in mice. Asian Pac. J. Cancer Prev. 10(6), 1089-94.
Sharma, P.V., 1998. Dravyaguna Vignana, Vol. II, Chaukhambha Bharati academy, Varanasi, India.
Shilpa, V.P., Muddukrishnaiah, K., Thavamani, B.S., Dhanapal, V., Arathi, K.N., Vinod, K.R., 2018. In vitro immunomodulatory, antifungal, and antibacterial screening of Phyllanthus niruri against to human pathogenic microorganisms. Environ. Dis. 3, 63-69.
Than, N.N., Fotso, S., Poeggeler, B., Hardeland, R., Laatsch, H., 2006. Niruriflavone, a new antioxidant flavone sulfonic acid from Phyllanthusniruri. Z. Natureforsch.61b, 57-60.
Tomaino, A., Cimino, F., Zimbalatti, V., Venuti, V., Sulfaro, V., De Pasquale, A., Saija, A., 2005. Influence of heating on antioxidant activity and the chemical composition of some spice essential oils. Food Chem. 89, 549-554.
Ueno, H., Horie, S., Nishi, Y., Shogawa, H., Kawasaki, M., Suzuli, S., 1988. Chemical and pharmaceutical studies on medicinal plants in Paraguay. Geraniin. An angiotensin-converting enzyme inhibitor from “praparai mi”, Phyllanthusniruri. J. Nat. Prod. 51, 357-359.
Useto, S., Kitagawa, Y., Kamishimoto, M., Kumagai, A., Hori, H., Nagasawa, H., 2001. Inhibition of green tea catechins against the growth of cancerous human colon and hepatic epithelial cells. Cancer Lett. 170, 41-44.
Venkateswaran, P.S., Millman, I., Blumberg, B.S., 1987. Effects of an extract from Phyllanthusniruri on hepatitis B and woodchuck hepatitis viruses. In vitro and in vivo studies. Proc. Natl. Acad. Sci. USA. 84, 274-278.
Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2018. Bioactive essential oils from the Cameroonian rain forest: A review - Part I. Trends Phytochem. Res. 2(4), 187-234.
Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2019. Bioactive essential oils from the Cameroonian rain forest: A review - Part II. Trends Phytochem. Res. 3(1), 3-52.
Weenen, H., Nkunya, M.H., Bray, D.H., Mwasumbi, L.B., Kinabo, L.S., Kilimali, V.A., Wijnberg, J.B., 1990. Antimalarial compounds containing an α,β-unsaturated carbonyl moiety from Tanzanian medicinal plants. Planta Med. 56, 371-373.
Whitley, A.C., Stoner, G.D., Darby, M.V., Walle, T., 2003. Intestinal epithelial cell accumulation of the cancer preventive polyphenol ellagic acid-extensive binding to protein and DNA. Biochem. Pharmacol. 66, 907-915.
Xu, H.X., Wan, M., Dong, H., But, P.P., Foo, L.Y., 2000. Inhibitory activity of flavonoids and tannins against HIV-1 protease. Biol. Pharm. Bull. 23, 1072-1076.
Yong, Li., Li, X., Wang, J.K., Kuang, Y., Qi, M.X., 2017. Anti-hepatitis B viral activity of Phyllanthusniruri L. (Phyllanthaceae) in HepG2/C3A and SK-HEP-1 cells. Trop. J. Pharm. Res. 16(8), 1873-1879.