Document Type : Original Article


1 Division of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow -226015 (U.P.), India

2 Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002(U.P.), India

3 Academy of Scientific and Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Sector-19, Kamla Neh Nagar, Ghaziabad-201002 (U.P.), India



Pluchea lanceolata, commonly known as Rasana, is highly valued for its anti-inflammatory, antiarthritic, and analgesic properties and is extensively employed in treating rheumatoid arthritis, cough, neurological diseases, edema, sciatica, psoriasis, bronchitis, dyspepsia, and piles. Shrinking populations and insufficient variation due to rootstock propagation methods warrant efficient mass multiplication and conservation protocols. In the present study, explant (axillaries bud was cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxin (PGRs) individually, as well as in combination. The highest mean shoot number (2.06 ± 1.43) and second highest mean shoot length (2.62 ± 1.49 cm) were obtained in MS media enriched with 4 mg/L Benzyl amino purine (BAP) + 1mg/L Naphthalene acetic acid (NAA). Whereas, the best shoots multiplication (2.62 ± 1.41) and shoots elongation (5.88 ± 2.44) responses were displayed when MS medium supplemented with 0.25 mg/L (BAP) +0.50 mg/L Kinetin (KIN) were used.

Graphical Abstract

In-vitro propagation of Pluchea lanceolata (DC) C.B.Clarke a potent antiarthritic medicinal herb through axillary bud


Agnihotri, R.K., Mishra, J., Nandi, S.K., 2009. Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et. Arn. Ex. Munro: production of genetically uniform plants and field evaluation. Acta Physiol. Plant. 8(2), 259-263.
Arya, D., Grover, N., Patni, V., 2008. Role of growth regulators in callus establishment and differentiation in Pluchea lanceolata (DC) C.B. Clarke. Plant Cell Biotech. Mol. Biol. 9, 127-134.
Arya, D., Patni V., 2013. Micropropagation of Pluchea lanceolate (Oliver &Hiern.)-A potent antiarthritic medicinal herb. Int. J. Med. Aromat. Plants 3, 55-60.
Espinosa, A.C., Pijut, P.M., Micher, C.H., 2006. Adventitious shoot regeneration and rooting of Prunus serotina in-vitro cultures. Hortic. Sci. 4, 193-201.
Gayathri, G., Arya, K., Bastian, D., 2009. Rapid multiplication of Fire Flamed Bush (Woodfordia fruticosa (L.) Kurz) through in-vitro techniques. Current Biotica. 2(4), 453-460.
Kanungo, S., Sahoo, S.L., 2011. Direct organogenesis of Withania somnifera L. from apical bud. Int. Res. J. Biotech. 2(3), 58-61.
Khare, C.P., 2004. Indian herbal remedies-rational western therapy, ayurvedic and other traditional usage, Botany. Berlin, Springer, 15-6.
Kher, M.M., Joshi, D., Nekkala, S.K., Nataraj, M., Raykundaliya, D.P., 2014. Micropropagation of Pluchea lanceolata (Oliver & Hiern.) using nodal explant. J. Hort. Res. 22(1), 35-39.
Kumar, S., Narula, A., Sharma, M.P., Srivastava, P.S., 2014. In vitro propagation of Pluchea lanceolata, a medicinal plant, and effect of heavy metals and different aminopurines on quercetin content in vitro. Cell. Dev. Biol. Plant 40,171-176.
Meena, M.K., Singh, N., Patni, V., 2011. In-vitro multiple shoot induction through axillary bud of Cocculus hirsutus (L.) Diels: A threatened medicinal plant. Afr. J. Biotechnol. 11(12), 2952-2956.
Murashige, M., Skoog, F., 1962. A revised medium for rapid growth and bioassay with Murugan, S., Mani, K. R., Devi, P. U., 2008. Prevalence of methicillin resistant Staphylococcus aureus among diabetes patients with foot ulcers and their antimicrobial susceptibility pattern. J. Clin. Diag. Res. 2, 979‐984.
Singh, B.P., 2004. Germplasm Introduction, Exchange, Collection/Evaluation and Conservation of Medicinal and Aromatic Plants-Their Export Potential, in Medicinal Plants Utilization and Conservation, Ed. by PC Trivedi, Aavishkar Publication, Jaipur, pp. 1-26.
Srivastava, P., Shanker K., 2012. Pluchea lanceolata (Rasana): Chemical and biological potential of Rasayana herb used in traditional system of medicine. Fitoterapia 83(8), 1371-1385.
Srivastava, V., Verma, N., Tandon, J.S., Srimal, R.C., 1990. Anti-inflammatory activity of Pluchea lanceolata: isolation of an active principle. Int. J. Crude Drug Res. 28,135-137.
Tiwari, V., Singh, B.D., Tiwari, K.N., 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L.) Wettst]. Plant Cell Rep. 17, 538-543.
Ullah, R., Bakht, J., 2016. Development of micropropagation protocol for medicinally important Periploca hispidus. Pak. J. Weed Sci.Res. 22(2), 253-261.
Uranbey, S., Cocu, S., Sancak, C., Parmaksız, I., Khawar, K.M., Mirici, S., Ozcan, S., 2003. Efficient adventitious shoot regeneration in Cicer milkvetch. Biotechnol. Biotec Eq. 17(1), 33-37.
Wang, J., Seliskar, D.M., Gallagher, J.L., 2004. Plant regeneration via somatic embryogenesis in the brackish wetland monocot Scripus robustus. Aquatic Bot. 79, 163-174.